?-Glucosidase Activity of Lactiplantibacillus plantarum UNQLp 11 in Different Malolactic Fermentations Conditions: Effect of pH and Ethanol Content
نویسندگان
چکیده
Lactiplantibacillus plantarum strain UNQLp 11 is a lactic acid bacterium with the potential to carry out malolactic fermentation (MLF) in red wines. Recently, complete genome of was sequenced and this possesses four loci enzyme ?-glucosidase. In order demonstrate that these glucosidase enzymes could be functional under harsh wine conditions, we evaluated hydrolysis p-nitrophenyl-?-D-glucopyranoside (p-NPG) synthetic different ethanol contents (0%, 12%, 14% v/v) at pH values (3.2, 3.5, 3.8). Then, precursor n-octyl ?-D-glucopyranoside analyzed sterile Pinot Noir (containing 14.5% v/v ethanol, values) by headspace sorptive extraction gas chromatography-mass spectrometry (HSSE-GC/MS). The p-NPG showed ?-glucosidase activity very susceptible low but induced presence high content. Furthermore, able release glycosilated n-octyl, during MLF greater extent than commercial enzyme. conclusion, improve aromatic profile volatile precursors MLF.
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ژورنال
عنوان ژورنال: Fermentation
سال: 2021
ISSN: ['2311-5637']
DOI: https://doi.org/10.3390/fermentation7010022